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pcdna3 1 mycbioid  (Addgene inc)


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    Structured Review

    Addgene inc pcdna3 1 mycbioid
    Pcdna3 1 Mycbioid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 112 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcdna3+1+mycbioid/pcDNA3%2E1+mycBioID+(Plasmid+%2335700)/pmc12824170-0-0-3
    Average 94 stars, based on 112 article reviews
    pcdna3 1 mycbioid - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: BioID-based intact cell interactome of the Kv1.3 potassium channel identifies a Kv1.3-STAT3-p53 cellular signaling pathway
    Article Snippet: .. The PCR product was digested by Xho I and Eco RI and inserted into pcDNA3.1 mycBioID (pcDNA3.1 mycBioID was a gift from K. Roux (Addgene plasmid no. 35700; http://n2t.net/addgene:35700 ; RRID:Addgene_35700). ..

    Plasmid Preparation:

    Article Title: BioID-based intact cell interactome of the Kv1.3 potassium channel identifies a Kv1.3-STAT3-p53 cellular signaling pathway
    Article Snippet: .. The PCR product was digested by Xho I and Eco RI and inserted into pcDNA3.1 mycBioID (pcDNA3.1 mycBioID was a gift from K. Roux (Addgene plasmid no. 35700; http://n2t.net/addgene:35700 ; RRID:Addgene_35700). ..

    Article Title: A novel role for the peptidyl-prolyl cis-trans isomerase Cyclophilin A in DNA-repair following replication fork stalling via the MRE11-RAD50-NBS1 complex
    Article Snippet: The requisite plasmids were obtained from AddGene ( https://www.addgene.org/ ); pcDNA3.1-MYC-BioID (Cat# 35700) and pcDNA3.1 MCS-BirA(R118G)-HA (Cat# 36047). .. The pcDNA3.1 mycBioID was a gift from Kyle Roux (Addgene plasmid # 35700; http://n2t.net/addgene:35700 ; RRID:Addgene_35700) and the pcDNA3.1 MCS-BirA(R118G)-HA was a gift from Kyle Roux (Addgene plasmid # 36047; http://n2t.net/addgene:36047 ; RRID:Addgene_36047). ..

    Clone Assay:

    Article Title: Forebrain Eml1 depletion reveals early centrosomal dysfunction causing subcortical heterotopia.
    Article Snippet: .. For BioID, full-length mouse Eml1 and Eml1*T243A cDNAs were cloned into the pcDNA3.1 mycBioID (#35700; Addgene) vector. ..

    Article Title: Forebrain Eml1 depletion reveals early centrosomal dysfunction causing subcortical heterotopia.
    Article Snippet: .. Full-length mouse Eml1 and Eml1*T243A cDNAs were cloned into pcDNA3.1 mycBioID (#35700; Addgene) vector using the following primers in Table 1. .. BioID (proximity-dependent biotin identification) analysis Mouse Neuro2A cells (CCL-131; ATCC) were transiently transfected 40 μg BioID constructs withp (PEI) (Cat. #23966; Polysciences Inc.) and after 24 h, cells were incubated overnight in the presence of 0.05 mM D-biotin (Cat. #B1595; Life Technologies), followed by cell lysis in a lysis buffer (150 mM NaCl, 0.5% SDS, 2% NP40, 1 mM EDTA, 10 mM IAA, 10 mM Tris pH 7.6, and protease inhibitor Cocktail, Cat.#11836170001; Roche).

    other:


    Article Title: BioID-based intact cell interactome of the Kv1.3 potassium channel identifies a Kv1.3-STAT3-p53 cellular signaling pathway.
    Article Snippet: To generate the mito- YFP- rKv1.3 construct, the mitochondrial targeting sequence of subunit VIII of cytochrome c oxidase amplified from pLV_mitoGFP (Addgene 44385) incorporating Nhe I–Age I restriction sites using Phusion High- Fidelity DNA polymerase (Thermo Fisher Scientific, F- 530) was inserted between Nhe I–Age I restriction sites (New England Biolabs, R3131 and R3552, respectively) in YFPrKv1.3 in pEYFP- C1 construct (from A. Felipe), using T4 Ligase (New England Biolabs, M0202).

    Amplification:

    Article Title: Styxl2 regulates de novo sarcomere assembly by binding to non-muscle myosin IIs and promoting their degradation
    Article Snippet: .. BirA* was amplified from pcDNA3.1 mycBioID (addgene, #35700) and an HA tag was added to its C-terminus. ..

    Article Title: ROS-dependent localization of glycolytic enzymes to mitochondria
    Article Snippet: Donor-derived fibroblasts were treated overnight with 1 μM MitoQ (New Zealand Health Manufacturing, RM0484C) diluted in absolute ethanol, or N-acetylcysteine (Sigma-Aldrich, A9165) prepared in PBS and pH adjusted to 7.4 with NaOH. .. To generate the fusion protein Myc-BirA∗-OMP25, the construct BirA(R118G) with Myc tag at the N-terminus was amplified from pcDNA3.1 mycBioID (a gift from Kyle Roux, Addgene #35700) using Myc-BirA∗ primers (5′-GCTAGCCGCCACCATGGAACAAAAACTCATCTCAG-3′ and 5′-CTCGAGCTTCTCTGCGCTTCTCAGGGAG-3′), which add Nhe I and Xho I restriction sites in forward and reverse, respectively. ..

    Construct:

    Article Title: ROS-dependent localization of glycolytic enzymes to mitochondria
    Article Snippet: Donor-derived fibroblasts were treated overnight with 1 μM MitoQ (New Zealand Health Manufacturing, RM0484C) diluted in absolute ethanol, or N-acetylcysteine (Sigma-Aldrich, A9165) prepared in PBS and pH adjusted to 7.4 with NaOH. .. To generate the fusion protein Myc-BirA∗-OMP25, the construct BirA(R118G) with Myc tag at the N-terminus was amplified from pcDNA3.1 mycBioID (a gift from Kyle Roux, Addgene #35700) using Myc-BirA∗ primers (5′-GCTAGCCGCCACCATGGAACAAAAACTCATCTCAG-3′ and 5′-CTCGAGCTTCTCTGCGCTTCTCAGGGAG-3′), which add Nhe I and Xho I restriction sites in forward and reverse, respectively. ..



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